Evaluation of a Nanobody-based competitive ELISA for the estimation of tsetse Oy bite exposure

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Willis A Adero
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Willis A Adero
Salivary proteins of hematophagous disease vectors can act as a potential biomarker of exposure to the bite of these insects, due to their ability to induce immunogenic/allergenic reactions in the host. This capability is important and can be explored for the development of serological tools that can be very useful of in entomological surveys and evaluation of impact assessment for already established control measures. Total salivary protein of the savannah tsetse fly Glossina morsitans morsitans (G.m.m) is a sensitive immunological probe that can be used to detect contact with tsetse flies. Nanobody based competitive ELISA using total G.m.m saliva have been developed and evaluated using mice and rabbit serum exposed to Glossina species and other hematophagous insects and the serological test have revealed that it is a sensitive and specific indicator that can be used as a risk marker of exposure to tsetse fly bite. Hence the test would be useful in the evaluation of intervention measure on tsetse control programmes and in the determination of exposure risk to tsetse fly bite.