Kenya Agriculture Research Institute Biotechnology Centre Annual Report 2007

No Thumbnail Available
Date
2007
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Kenya Agriculture Research Institute
Abstract
This is a two prong linked project focusing on identification and use of Bacillus thuringiensis (Bt) for the control of Prostephanus truncatus (Pt) and Sitophilus zeamais (Sz) and, Agro bacterium-mediated transformation of elite Kenyan maize germplasm for the control of the two insect pests. Very little progress has been realised with the two projects. Of the several new Bt colonies isolated, seven were screened for insecticidal activity and showed lack of good control of Pt and Sz. One inbred, CML216, demonstrated highest transformation frequency when crossed with maize inbred A 188. Crosses made between T04 inbred and 1004 inbred with A188 also yielded mature plants. Implementation of these projects has been constraint by persistent delay in procurement of laboratory supplies/reagents. Efforts initiated to link up with public/private sector for the purposes of accessing effective crude Bt proteins, gene constructs and specific grain promoters were unsuccessful.
Description
Keywords
Citation
Collections