Isolation Of A Theileria Species From Eland Infective for Cattle

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Kenya Agriculture Reserch Institute
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Kenya Agriculture Reserch Institute
Theileria infections were ind uced in cattle by feeding ticks on them from 3 sources: (a) adult rhipicephalid ticks obtained from the vegetation in a paddock-confiiiningiin- eland EAO at the Animal Orphanage, Nairobi National Park, Kenya, (b) Rhipicephalus appendiculatus adults fed as nymphs on-t-he same eland, (c) R.-pulcnellus adults fed as nymphs on an eland W 68 captured in the Machakos district of Kenya. Both eland were harbouring Theileria parasItes at the time nymphal tIcks were fed. Mild infections were pro duced when adult ticks from these 3 batches were applied to cattle associated with low numbers of schizonts and piroplasms. The indirect fluorescen t antibody test demonstrated that cattle recovered from mfections resultmg from the above 3 tick batches from eland W 68 and EAO produced antibodies which reacted with schIzont antigen of the Theileria species (eland) and Theileria species (Githunguri) which had been Isolated from cattle and not to antigens of other Theileria species used. The cattle recovered from the Theileria species (eland) were fully susceptible to a lethal challenge of a T. par va( Muguga) stabilate