Characteriza Tion Of Colletotrichum Orbiculare And Allied Species Using Mtdna Rflps, Vegetative Compatibility, And Virulence On Cucurbits, And Screening Cucumber And Watermelon For Anthracnose Resistance

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Date
1997
Authors
Lusike W.
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Lusike W.
Abstract
Based on spore morphology, appressorium development, sequence similarities of the rDNA and similarities in amplified restriction fragment length polymorphism (AFLPs), others have concluded that C. orbiculare, C. trifolii, C. lindemuthianum, and C. malvarum represent a single phylogenetic species. In this study, several representative isolates of races 1, 2, and 2B of C. orbiculare, representing four vegetative compatibility groups (VCGs), and several isolates of C. trifotii from alfalfa, C. lindemuthianum from green bean, and C. malvarum from common mallow and prickly sida were examined for mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs), vegetative compatibility, and host specificity on cucurbits in inoculation experiments on four differential cucurbit cultivars. Isolates of C. orbiculare, C. trifolii, and C. malvarum had an identical mtDNA haplotype (haplotype A) when examined with three different restriction enzymes. Isolates of C. tindemuthianum had a very similar mtDNA haplotype to haplotype A, with a single polymorphism detected with the enzyme Haeili. Isolates of C. trifolii belonged to a single VCG as did isolates of C. malvarum,
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