Development Of An In Vitro Technique For Cytological Investigations Of Slices Of

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Date
1975
Authors
HANNA B
THREADGOLD L. T.
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TIIRrADGOLD L. T. and HANNA R. B. 1975. Development of an ivitro technique for cytological investigations of slices of Fasciola hepatica: evaluation by physiological criteria. /Ilterllatiollal JOl/rural for Parasitology 5: 333337. In order to check the viability of Fasciola hepatica sllccs, maintained in M 199 under defined physio-chcmical conditions, their rates of consumption of oxygen and glucose were determined, throughout a 12-h period, using polarographic and spectrophotometric techniques. The results, when compared with similar determinations on intact animals, indicated that the slices remained as viable as incubated whole flukes, for at least 12 h. This finding supports ultra structural evidence detailed in a previous~ publication. The slices were shown to consume more oxygen and less glucose than whole animals, and possible reasons for this are discussed. Studies on the effects of DNP and iodoactate on the uptake behaviour of slices suggested that In Fasciola hepatica, a~ in aerobes, most of the oxygen and glucose consumed is involved with energy metabolism. Hence, oxygen and glucose uptake rates arc probably valid criteria of tissue viability for the ~Iiccs.
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East African Agricultural And Forestry Journal, 5, p. 333-337
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