Electrophoresis as an Aid for Identification Of

Thumbnail Image
Sudu, S.
Journal Title
Journal ISSN
Volume Title
Starch gel electrophoresis was conducted on three enzymes (peroxidase, esterase and leucine aminopeptidase) from the 12 cultivars of Amaranthus caudatus, A. hypochondriacus and A. cruentus. Best zymograms were obtained when the plants were 60 days old. At this stage, on the basis of peroxidase, A. cruentus could be identified because it did not have anodic bands A3 and A4 • Band A3 was specific to A. caudatus whereasA. hypochondriacus could be distinguished fromthe rest because it did not have band A3 but had only one light staining cathodic band (C1). On the basis of esterase, each species could be distinguished as follows: band A4 was specific to A. cruentus, band As specific to A. hypochondriacusand bands As and A9 coalesced and had dark staining specific to A. caudatus. On the basis' of leucine aminopeptidase, A. cruentus could be distinguished because it produced this enzyme earlier and had a higher quantity of the enzyme than the other two species. The zymograms of leucine aminopeptidase and peroxidase showed close banding homology between A. hypochondriacus and caudatus. The esterase zymogram, however, showed some slight banding homology between A. hypochondriacus and A. cruentus. It could be suggested on the basis of electrophoretic banding homologies that two species A. hypochondriacus and A. caudatus are phytogenetically closely retated and the same could be said for A. hypochondriacus and A. cruentus. However, no relationship was established between A. cruentus and A.caudatus.
East African Agricultural And Forestry Journal, 52 (4), pp. 244-250