A Plaque assay system Rinderpest Virus and Its Use in Characterising Virus Adsorption

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Taylor, W.P
Perry, C.T
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A continuous line of calf kidney cells was found suitable for the production of plaques by five strains of rinderpest virus known to differ in their virulence for cattle. No differences were seen in the morphology of the plaques produced by these strains. Plaques were first seen after 6 days incubation, and if neutral red was incorporated in the overlay appeared as "red" centres composed of a small cluster of cells taking up this stain more strongly than the healthy background cells; thereafter plaques enlarged very slowly and were only 1.5-2.0 mm in diameter after 14 days. Plaque counts did not increase beyond the 6th day of incubation and were unaffected by either agarose concentrations of 0.4 to 1.5% or overlay pH values between 6.7 and 7.85. It was shown that neutral red included in the initial overlay did not affect the sensitivity of virus titrations.
East African Agricultural And Forestry Journal, p. 270-282