Infection and Transformation of Bovine, Lymphoid Cells in vitro by Infective Particles of Theileria parva

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Date
1973
Authors
Brown C.G.D
Stagg D.A
Purnel R.E
Kanhai G.K
Payne R.C
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Abstract
Technique; recently developed in this laboratory can be used to infect cattle reproducibly with East Coast fever with infective particles qf Theileria parva collected from the tick vector, Rhipicephalus appendiculatlls. Infective particles can be COllected1'ither as in vitro tick feed material by inducing prefed, infected ticks to salivate into capillary tubes containing foetalcal serum!, or as ground tick supernatant obtained by grinding he ticks in Eagle's minimum essential medium with Earle's s ts (MEM) supplemented with bovine plasma:! Albumin (Fraction: V from bovine plasma) (BPA) and collecting the supernat~nt2
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Citation
East African Agricultural And Forestry Journal, 245, p. 101-103
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