Studies on Isometamisium Chloride; Pharmacokinetics and Efficacy Against Trypanosomiasis in Sheep and Goats

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Date
2000
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Kenya Trypanosomiasis Research Institute
Abstract
The work described in this thesis was carried out on sheep and goats under natural tsetse and controlled trypanosome challenge conditions. An initial study was carried out at Kiboko range station Kenya to determine serum isometamidium concentration in sheep and goats under natural tsetse challenge conditions, which had been treated intramuscularly with isometamidium chloride at Imglkg b.w. Serum isometamidium concentrations were determined using the isometamidium enzyme linked immunosorbent assay and all the animals were monitored weekly for trypanosome infection and PCV. Tsetse fly numbers were monitored using six biconical traps. The results from this study indicated that the isometamidiumELISA developed for cattle could be used successfully to determine isometamidium concentration in sheep and goats. Isometamidium levels were observed to decline in two phases in both sheep and goats, a rapid phase followed by a slow phase. Isometamidium was detectable by ELISA for up to 98 days post treatment in both species. However, no trypanosome infections were seen in either the isometamidium treated or untreated control sheep and goats. This absence of infection was probably related to the absence of trypanosome challenge as no tsetse flies were trapped throughout the period of study. Consequently, it was impossible to relate serum isometamidium concentration to protection against infection. Subsequently, further studies were carried out under controlled challenge conditions
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