Kenya Agriculture Research Institute Biotechnology Centre Annual Report 2004

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Kenya Agriculture Research Institute
Simple sequence repeat (SSR) markers were used to study recombinant inbred lines originating from a single cross between two progenitor inbred lines OSU 23i and EMI2- 210, for resistance to MSV. A total of 115 recombinant inbred lines belonging to three families were screened using 52 SSR markers contained between Bin 1.04 and 1.05 of chromosome 1. These markers were reduced to only one SSR marker on the basis of their polymorphism content. This paper reports on the results of selection by use of artificial inoculation and one selected SSR marker contained in Bin 1.05 of chromosome 1. Selection by the use of artificial inoculation alone detected 78 resistant lines and 37 susceptible lines. On the other hand, using the SSR marker alone detected 40 resistant lines, 64 susceptible lines (both heterozygotes and homozygous dominant), and 11 were not scored. But a combination of both the artificial inoculation and SSR marker selection detected 29 resistant and 75 susceptible lines, while the rest 11 lines were discarded. With these findings we concluded that, a conventional maize breeder requires the use of molecular markers in order to improve selection intensity and maximize genetic gain. The SSR genotypic data were not analyzed as there was no software for windows. However, these data will later be analyzed by association mapping using comparative map and trait viewer (CMTL).