Trilayl 2010

Loading...
Thumbnail Image
Date
2010
Authors
Ndungu, K
Journal Title
Journal ISSN
Volume Title
Publisher
Ndungu, K
Abstract
The efficacy of a bull cryopreservation medium Triladyl® in preservation of the various pathogenic trypanosomes was evaluated against the commonly used glycerol ethylenediamine tetraacetic acid (EDTA) saline glucose (ESG). Trypanosoma brucei rhodesiense, T. evansi, T. b. brucei and T. congolense were first propagated in Swiss White mice. At peak of parasitemia, blood was collected from the mice by cardiac puncture and 1 x 102 trypanosomes/ml dilution made. This dilution was then divided into 2 equal portions which were cryopreserved in either ESG 10% glycerol or Triladyl® 50%. After 1 month of cryopreservation, 2 irradiated mice were intraperitoneally administered with 0.2 ml of the cryopreserved sample and then monitored for parasitemia development.
Description
Keywords
Citation
Collections