ROK Kenya Trypanosomiasis Research Institute Annual Report 1981

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Kenya Trypanosomiasis Research Institute
In lower vetebrates, such as the smooth dogfish, Mustelus canis and the lemonshark Negaprion brevirostris (Clem and Small Jr., 1967) antigenic stimulation elicits the formation of both low molecular weight (7 S IgML) and high molecular weight (19 S, IgMN) immunoglobulin M both belonging to the J1. heavy chain class. The induction of these molecular species has been demonstrated in horses immunised with pneumococci (Sandor, Korach and Mattern, 1964) and in humans, by Killander (1963) when he examined sera from persons containing various blood group antibodies and pathological sera from patients with myeloma and macroglobulinaemia. Since then, the two molecular species have been demonstrated in a number of disease states (mainly Iymphoproliferative) and in parasitic infections including human and animal trypanosomiasis (Frommel, Perey, Masseyeff and Good, 1970). Quantitative and qualitative studies on serum IgM do not usually take into account the effect of the coexistence of the two molecules. Using human sera, therefore, the two molecules were isolated on sucrose gradients in the ultracentrifuge. In this report the effect of 7 S on 19 S 1 gM was investigated with respect to two tests which are some of the routinely used diagnostic tests in African Trypanosomiasis, viz: (a) The indirect immunofluorescent antibody test. (b) The radial immunodiffusion test.