Dual Occurrence of Cassava Mosaic Geminiviruses And

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Date
2013
Authors
Irungu, J.
Ngeranwa J.J
Miano, W.D
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Abstract
Viral diseases, especially cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) cause major economic significance leading to poor cassava yields. The CMD is widely distributed in the cassava growing regions while new reports of occurrence of CBSD at high altitude areas of Western Kenya have emerged. In an attempt to investigate the dual complexity of the two viruses in western Kenya, 217 cassava samples were randomly collected from 77 farmer's fields at predetermined intervals along motorable roads in five districts of western Kenya and subjected to endpoint polymerase chain reaction (PCR) and reverse transcriptase polymerase chain reaction (RT- PCR) for detection of CMGs (Cassava Mosaic Geminiviruses) and CBSV (Cassava Brown Streak Virus) respectively. There were differences (P<O.OS) in percentage mean incidence in the occurrence of CMD (84%) and CBSD (65%). The overall CMD and CBSD dual incidence among the landraces was significant (P<O.OS) in Adhiambolera (4S %) and Serere (65%) while not significant (P<O.OS) in Embwanatereka (S2%) and Kibandameno (41%). Among the improved varieties encountered, CMD incidence (64%) was lower compared with the landraces but CBSD incidence (55%) was comparable to the landraces. The other less popular local varieties sampled had incidence of 78% and 49% for CMD and CBSD respectively. These results confirm the dual occurrence of CMD and CBSD in all the districts surveyed in Western Kenya, infecting the local popular varieties, as well as the less popular local and improved varieties
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East African Agricultural And Forestry Journal, 79 (3), p. 133-141
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