Coffee Board of Kenya Monthly Bulletins August 1990

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1990
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The experiment was started with an aim of finding out the possibility of using tissue culture as a tool for propagating the improved cultivar obtained in the breeding programme.Mature leaf ex plants of Coffea arabica L cultivar Ruiru 11 were cultured in a Murashige and Skoog (MS) (1962) nutrient medium with varying concentration of auxins and cytokinins for callus proliferation. Embryogenesis and plantlet development was achieved on a media containing same formulation with reduction in the MS organic salts and 2,4D replaced with 0.8 mg IAA. These preliminary results indicate that tissue culture is a promising tool for propagating Coffea arabica L.
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Bulletin, 55 (646), pp. 897-912
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