Identification of the Origin of Animal Protein by Agar-gel Diffusion

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Date
1962
Authors
Hay D.
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Abstract
THE identification of the origin of various animal proteins proved possible by the agar-gel double diffusion technique. Antisera were prepared in rabbits by multiple injections of alum-precipitated animal sera. Antigens were prepared from animal sera and tissue extracts, tick blood-meals, sun-dried moat or biltong and uncooked sausage meat. They wore diffused overnight at 25° C against known antisera simultaneously with the homologous antigen_ The agar gels wore prepared from 1 per' cent agar in borate buffer at pH 8•6 and were about 3mm thick. A seven-well pattern was used consisting of a 9,5mm diameter control well set 10 mm from the 7 –nun diameter peripheral antigen wells.
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Nature, 196 (4858)
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