Identification of Amplified Fragment Length Polymorphism (AFLP) Markers Linked To Maize Streak Virus (MSV) Disease Resistance Genes

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Lagat, M.K.
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Martin K.L
Maize (Zea mays L.) is the most important cereal in Sub-Saharan Africa, where it is used as human food animal feed, raw material for various industrial products and as source of employment at various stages of production. In Kenya, lack or limited availability of maize is equated to hunger. Despite its significance, the average annual production remains lower than the average annual consumption. One of the contributors to low productivity of this important crop are diseases, particularly maize streak virus which causes up to 100% yield loss. The disease is difficult to control through conventional methods and therefore, use of resistant varieties is a more feasible method of managing it. However, the conventional methods of breeding and deploying resistant varieties take a long time, and are dependent on crop growth stage and prevailing environmental conditions. Thus, there is need for better tools and techniques such as DNA-based molecular marker assisted selection. The objective of this study was to map AFLP regions linked to maize streak disease resistance in two single crosses arising from inbred lines, OSU23i (resistant), CML 202 (resistant), and EM12-210 (susceptible). Phenotypic screening of the resistant parents revealed inbred line OSU23i was more resistant with mean score of 1.13, and CML 202 had a mean score of 1.21. The susceptible parent EM12-210 interestingly had a low mean score of 1.33 which was close to the resistant parents. The average Fl score was 1.5, which was close to the midparent value of 1.22. The average F2 score was 1.92, which, was 0.42 units less resistant than the Fl and 0.7 units less resistant than the mid-parent.