In Vitro manipulation Of Cloned Amidase Genes Of Pseudomonas Aeruginosa

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Date
1988
Authors
Daniel K M
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Daniel K M
Abstract
The species of the genus Pseudomonas are able to utilise a '. wide range of organic compounds as growth substrates. The ini tial attack is carried out by inducible enzymes and lat e -r intermediates enter the reactions of central metabol i c pathways ( Clarke, P.H.,1973 ). Pseudomonas aeruginosa utilises acetamide and propionamide via the activity of an inducible aliphatic amidase ( acyl amidohydrolase, EC . 3.5.1.4. , Kelly and Clarke,1962; Brammar 1and Clarke,1964 ). The aliphatic amidase gene which is required fo r this process is chromosomally located and its expression is inducible by both substrate and various non-substrate ami des ( Brammar and Clarke, 1964
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